diff --git a/gstreamer/mediaobject.cpp b/gstreamer/mediaobject.cpp
--- a/gstreamer/mediaobject.cpp
+++ b/gstreamer/mediaobject.cpp
@@ -331,6 +331,10 @@
         QString absCompleteBaseName = m_source.fileName();
         absCompleteBaseName.chop(QFileInfo(absCompleteBaseName).suffix().length());
 
+        // clean previously associated subtitle
+        g_object_set(G_OBJECT(m_pipeline->element()),
+            "suburi", NULL);
+
         // Looking for a subtitle in the same directory and matching the same name
         foreach (const QString &ext, exts) {
             if (QFile::exists(absCompleteBaseName + ext)) {